Trinetrudu movie is Chiranjeevi's 100th film, this is an action oriented movie in which, Goa's beach is being exploited by a big-wig in the society, named D.D. He runs a big Aashram on Goa's beach, and under this Aashram's mask, he runs his underworld. His underworld is the channel for drug-trafficking, which cashes upon the weakness of the youth.
To investigate about this Aashram's dark secrets, Indian government appoints a CBI officer (Nagendra Babu), who prepares a complete report on its activities, but is killed by D.D's men.
When CBI officer never returns, CBI gets suspicious and sends another officer, who is Abhimanyu (Chiranjeevi). Abhimanyu lands in Goa and meets D.I.G (Kaikala Satyanarayana) to inform him about his mission. But Goa police and CBI doesn't share good terms and D.I.G appoints 2 Sub Inspectors(NuthanPrasad and Prasad babu) to follow and observe Abhimanyu. Meanwhile, Bhanupriya's brother becomes a drug addict, and to cure him she gets him admitted into a hospital.
Hospital chief Ranganath meets Abhimanyu in a strange incident. When bhanu's brother tries to jump off the top floor of hospital, Abhimanyu saves him and thus meets bhanu. Rest of the movie is about how Abhimanyu put an end for D.D.