Gambeeram movie is all about Assistant Commissioner of Police Muthusamy (Sarath Kumar) who is an honest cop who fights for justice. His wife Saroja (Pranathi) dies while giving birth to their daughter and Sarath lives the life of a widower with his little daughter Krithika (Baby Monica).
Vijayalakshmi (Laila) is a constable who reports to him. She pours motherly love on the girl and also desired to become her mother.
Meanwhile, Muthusamy arrests illicit liquor brewer Naga (Jasper) and earns the wrath of 3 VIPs- industrialist Punniyakoti (Sethu Vinayagam), Central Minister Rajendran (Thanikkal Bharani) and IAS Officer Manibharathi (Manikka Vinayagam).
The rest of the story is about how he deals with these VIPs.