Twinkle Twinkle Little Star movie based on the life of a child in a royal family headed by a matriarch who lives in the palace with her two daughters (Swetha and Pooja ). Swetha is married and has a son named Chintu while Pooja is an IPS Officer.
Chintu is found to have a non curable physical deformity and it is proclaimed that the child will grow as a eunuch in future. This news was a great shock to the family and they are forced to go behind the spiritual remedies to rescue the child from growing into a eunuch. From the advise of the spiritual practioners the family of chintu came to know that Chintu was born as a eunuch because of the curse of the kuludevata and they were asked to perform poojas in the haveli by a eunuch priest to ward off the evil.
For performing poojas they approach the head of the eunuchs name "Kakathiya" who is supposed to be 200 years old and deeply believes in powers and his ongoing research to transform eunuchs in to powerful human beings. His belief was high that one day he would be successful in his research works, transforming eunuchs to super human beings and to rule the world.
Kakathiya sends his beloved disciple and priest Chela and his aide to the haveli to perform the poojas. At the palace, a new relationship sprouts between Chela and Chintu which threatens Swetha. Even after taking many security measures the relationship kept on growing between Chela and Chintu.
From here the story moves into thrilling and chilling twist and turns. A series of murders occurs and Pooja, the IPS officer is assigned to investigate and she founds that all those who were murdered were young virgins, which leads into strange rituals and practices. The story turns in to a excellent experience of mystery, mystique, horror and final victory.